RNA fingerprinting by molecular indexing
نویسندگان
چکیده
منابع مشابه
RNA fingerprinting by molecular indexing.
Class IIS restriction enzymes, a subgroup of class II, cleave DNA at a precise location outside their recognition sites, and produce overhangs of unknown sequences (1). Molecular indexing is a series of techniques designed to characterize DNA fragments by these unknown sequences (2–4). I applied this principle for description of the total mRNA population using a 3′ end cDNA fragment generated b...
متن کاملGeodabs: Trajectory Indexing Meets Fingerprinting at Scale
Finding trajectories and discovering motifs that are similar in large datasets is a central problem for a wide range of applications. Solutions addressing this problem usually rely on spatial indexing and on the computation of a similarity measure in polynomial time. Although effective in the context of sparse trajectory datasets, this approach is too expensive in the context of dense datasets,...
متن کاملElectronic fingerprinting of RNA.
Software has been developed to assist RNA fingerprinting analysis. One program generates, from a DNA sequence data file, the oligonucleotides resulting from digestion of an RNA transcript labeled with any specified nucleotide(s). Oligonucleotides are sorted according to their position on the fingerprint. Expected molar yields and products of secondary redigestion are also indicated. A second pr...
متن کاملAccurate and predictive antibody repertoire profiling by molecular amplification fingerprinting.
High-throughput antibody repertoire sequencing (Ig-seq) provides quantitative molecular information on humoral immunity. However, Ig-seq is compromised by biases and errors introduced during library preparation and sequencing. By using synthetic antibody spike-in genes, we determined that primer bias from multiplex polymerase chain reaction (PCR) library preparation resulted in antibody frequen...
متن کاملPhysico-chemical fingerprinting of RNA genes
We advance here a novel concept for characterizing different classes of RNA genes on the basis of physico-chemical properties of DNA sequences. As knowledge-based approaches could yield unsatisfactory outcomes due to limitations of training on available experimental data sets, alternative approaches that utilize properties intrinsic to DNA are needed to supplement training based methods and to ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 1362-4962
DOI: 10.1093/nar/24.2.394